Journal: Mucosal immunology

Article Title: Regulatory T cells protect against aberrant remodeling in a mouse model of pulmonary fibrosis

doi: 10.1016/j.mucimm.2025.12.004

Figure Lengend Snippet: Regulatory T cell dynamics in lungs during fibrosis. (A) The previously described SP-C I73T mouse pulmonary fibrosis model was utilized with an induction protocol containing 2 sequential doses of tamoxifen delivered by oral gavage that produces 2 distinct phases of overt disease development – alveolitis or peak inflammation (1–2 weeks) and fibrogenesis or fibrotic remodeling (2–6 weeks); (B) Lungs from control and SP-C I73T mice were analyzed for Foxp3+ Tregs by flow cytometry at indicated time points post Tam induction. Data pooled from three independent experiments with a total of 3–12 mice in each group as presented in the scatter plots. Kruskal-Wallis multiple comparison one-way ANOVA was performed. *** p = 0.0006 and **** p < 0.0001; (C) Representative immunohistochemical staining of paraffin-fixed SP-C I73T lungs obtained 4-weeks post induction and stained for murine Foxp3 (mFoxp3) expression. Arrows indicate mFoxp3+ Tregs localized in perivascular and fibrotic areas, high power magnification of insets 1,2. Bar: a = 100 μm, b-d = 10 μm,1–2 = 10 μm; Images are representative of 3–5 animals per group; (D) Uniform manifold approximation and projection (UMAP) analysis of 1,341 CD3+ T cells from lung scRNAseq data ( GSE234604 ) reveals a Foxp3+ Treg cluster following mutant SP-C expression. Gradient dot plots show expression of Treg-specific genes Foxp3 and Il2ra in SP-C I73T mice at 2- and 4-weeks post tamoxifen induction (n = 2 per genotype per time point); (E) Representative immunohistochemical staining for human Foxp3 (hFoxp3) in a paraffin embedded section from a human IPF lung explant. Insets 1–4 are magnified views of boxed regions. Bar 1–4 = 100 μm, a = 1000 μm (F) UMAP visualization of 8,404 CD3+ CD4+ T cell subtypes from lung cells analyzed by scRNA-seq in accession number GSE227136 , comparison of relative frequency of Treg (Foxp3+) clusters between control (n = 12) and IPF (n = 17) patients, a threshold cut-off > 50 cells was used for analysis; ** p = 0.004 by two-tailed Mann-Whitney test.

Article Snippet: To generate an I ER -SP-C I73T -Foxp3 DTR knock-in mouse line, we used B6.129(Cg)- Foxp3 tm3(Hbegf/GFP)Ayr /J purchased from The Jackson Laboratory (strain # 016958).

Techniques: Control, Flow Cytometry, Comparison, Immunohistochemical staining, Staining, Expressing, Mutagenesis, Two Tailed Test, MANN-WHITNEY

Journal: Mucosal immunology

Article Title: Regulatory T cells protect against aberrant remodeling in a mouse model of pulmonary fibrosis

doi: 10.1016/j.mucimm.2025.12.004

Figure Lengend Snippet: Regulatory T cells in fibrotic lungs have increased expression of effector and activation markers (A) Foxp3 eGFP reporter mice were crossed with previously published SP-C I73T mice to generate SP-C I73T - Foxp3 eGFP mice; (B) CD4+ Foxp3-GFP+ T regs were analyzed by flow cytometry in lungs harvested at 2, 3 and 4 weeks post Tamoxifen induction of mutant SP-C. Ordinary one-way ANOVA was performed. ** p < 0.005 (n = 3–5 mice in each group); (C-G) Control and SP-C I73T -Foxp3 eGFP lungs were digested, and samples were enriched for T cells using magnetic bead depletion as described in . Flow cytometric analysis and quantification of geometric mean fluorescence intensity of effector and activation markers expressed by CD4+ Foxp3-GFP+ T regs in lung tissue of SP-C I73T -Foxp3 eGFP mice 2 and 4-weeks post mutant Sftpc induction: (C) CD44; (D) CD279 (PD-1); (E) CD103; (F) GITR; (G) TIGIT. For each marker, ordinary one-way ANOVA was performed and *p < 0.05 and ** p < 0.005, n = 3–5 mice per group.

Article Snippet: To generate an I ER -SP-C I73T -Foxp3 DTR knock-in mouse line, we used B6.129(Cg)- Foxp3 tm3(Hbegf/GFP)Ayr /J purchased from The Jackson Laboratory (strain # 016958).

Techniques: Expressing, Activation Assay, Flow Cytometry, Mutagenesis, Control, Fluorescence, Marker

Journal: Mucosal immunology

Article Title: Regulatory T cells protect against aberrant remodeling in a mouse model of pulmonary fibrosis

doi: 10.1016/j.mucimm.2025.12.004

Figure Lengend Snippet: Depleting T regs during fibrogenesis worsens the disease phenotype in I ER -SP-C I73T -Foxp3 DTR mice. (A) I ER -SP-C I73T -Foxp3 DTR knock-in mice generated as described in . SP-C I73T -Foxp3 DTR mice were first induced with Tamoxifen administered via oral gavage on Days 0 and 4. 5 μg of DT or PBS was then administered i.p. every 48 h starting at day 17 post Tam induction and weight loss was recorded every day in SP-C I73T and SP-C I73T -Foxp3 DTR mice. Data are pooled from three independent experiments. ***p < 0.0001 by two-way ANOVA; (SP-C I73T uninduced – no tam+ DT n = 10, SP-C I73T + DT n = 16, SP-C I73T - Foxp3 DTR + PBS n = 13, SP-C I73T -Foxp3 DTR + DT n = 27); (B) Kaplan Meier survival curves of SP-C I73T -Foxp3 DTR mice that either received PBS (n = 13) or DT (n = 25). Log-rank (Mantel-Cox) test was performed with p value 0.0391; (C) Lungs from SP-C I73T -Foxp3 DTR mice receiving DT (n = 9) or PBS (n = 9) were harvested and cell suspensions analyzed by flow cytometry for GFP+ Tregs. *** p value < 0.0001 by unpaired t -test with Welch’s correction; (D) Quantification of total BALF cell counts between SP-C I73T -Foxp3 DTR mice treated with PBS (n = 7) or DT (n = 12) 26 days after Tam induction. * p = 0.01 by unpaired t -test with Welch’s correction; (E)(Left) Representative Picrosirius red stained fields from lung sections from DT or PBS treated SP-C I73T -Foxp3 DTR mice 26 days after Tam induction. Bar = 200 μm. (Right) Quantification was performed using ImageJ and data expressed as a percentage area staining for PSR. ** p = 0.004 by unpaired t -test with Welch’s correction; (F) BALF TGFβ quantified using ELISA (PBS n = 11, DT n = 11); (G) qRT-PCR analysis of whole lung mRNA for Col3a (PBS n = 12, DT n = 8); For E- G, scatter plots with individual values data was analyzed by unpaired t -test *p < 0.05 ** p < 0.01; (H) Box and Whiskers plot of static lung compliance (Cst) measured using Flex-Vent as described in (PBS n = 11, DT n = 12). Individual values for each animal are depicted and analyzed by unpaired t -test *p < 0.05 ** p < 0.01.

Article Snippet: To generate an I ER -SP-C I73T -Foxp3 DTR knock-in mouse line, we used B6.129(Cg)- Foxp3 tm3(Hbegf/GFP)Ayr /J purchased from The Jackson Laboratory (strain # 016958).

Techniques: Knock-In, Generated, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: Mucosal immunology

Article Title: Regulatory T cells protect against aberrant remodeling in a mouse model of pulmonary fibrosis

doi: 10.1016/j.mucimm.2025.12.004

Figure Lengend Snippet: Tregs in SP-C I73T mice display a type 2 signature post injury. (A) Reanalysis of a previously published and deposited scRNAseq data set ( GSE234604 ). (Top) UMAP clustering of 1,341T cells in SP-C I73T and uninduced control mouse lungs and an accompanying gradient plot depicting genes used for their classification (Lower) identifies identified 2 populations of Foxp3+ T regs as proliferating Gata 3 low and activated Gata 3 high plus CD4+ and CD8+ T cells at 2- and 4-weeks post injury; (B)) UMAP projection T cell populations identifies the non-proliferating Foxp3 Treg population as expressing Gata3 but low in Tbx21 and Rorc; (C) Gradient plot depicting Gata3-regulated genes – in lung Tregs at 2 and 4 weeks post-injury and uninduced control (“WT”) mice; (D) Representative FACS plot of lung cell suspension obtained 3 weeks post-Tam induction showing CD25hi Foxp3+ T regs co-stained for transcription factors – Tbet (Th1), GATA3 (Th2) and Rorgt (Th17) as labeled. Spleens (n = 3) from of SP-C I73T animals served as controls. Unpaired t -test was performed and *p = 0.01; (E) Lungs from uninduced control (“WT”) and SP-C I73T mice were analyzed and quantified by flow cytometry for transcription factors – Foxp3 and GATA3 flow cytometry at indicated time points post Tam induction. Ordinary one-way ANOVA was performed. * p < 0.05 ** p < 0.005. n = 3–5 mice per group; (F) Comparative analysis of deposited scRNAseq data set ( GSE234604 ) for genes associated with Th2 immune response 4-weeks post Tam induction. Shown are data from uninduced control (“WT”) and SP-C I73T mice represented in a gradient plot. Genes from the gradient plot were combined to generate a Th2 gene score calculated for T regs from SP-C I73T and uninduced control mouse lungs.

Article Snippet: To generate an I ER -SP-C I73T -Foxp3 DTR knock-in mouse line, we used B6.129(Cg)- Foxp3 tm3(Hbegf/GFP)Ayr /J purchased from The Jackson Laboratory (strain # 016958).

Techniques: Control, Expressing, Suspension, Staining, Labeling, Flow Cytometry

Journal: Mucosal immunology

Article Title: Regulatory T cells protect against aberrant remodeling in a mouse model of pulmonary fibrosis

doi: 10.1016/j.mucimm.2025.12.004

Figure Lengend Snippet: T regs in SP-C I73T mice are proliferative and make pro-reparative factors. (A) Schematic for two dose Tamoxifen induction (days 0 and 4) of I ER -SP-C I73T -Foxp3 eGFP mice. Foxp3-GFP+ Tregs were FACS purified at 2-, 3- and 4-weeks post Tam induction and total RNA isolated. (B-D) Scatter plots of qRT-PCR analysis of Treg mRNA for expression of: (B) Ki67; (C) Top2a (Topoisomerase2a); (D) Areg. For each gene ordinary one-way ANOVA was performed. * p < 0.05 *** p < 0.0001. n = 3–7 mice per group; (E) Foxp3-GFP+ Tregs were FACS sorted from lungs and spleen of SP-C I73T mice 4 weeks post Tam induction and plated overnight. Cells were then treated with a cell stimulation cocktail that includes Brefeldin A, a protein transport inhibitor for 4 h, fixed and stained for GATA3 and intracellular amphiregulin (Areg). Identified Gata3+/Areg+ cells were expressed as a percentage of total Tregs. Comparisons were made using an unpaired t -test. *p = 0.01 n = 3–8 mice per group; (F) Areg levels of BALF collected 4wks after Tam induction and quantitated by ELISA. ***p = 0.0002 by unpaired t -test; n = 7–8 per group; (G) Gradient plots from the previously published scRNA-seq dataset GSE234604 were re-analyzed to compare the expression of Areg, GATA3 and Ki67 among Tregs, proliferating Tregs, CD4 + , and CD8 + T cells identified in single cell suspensions prepared from lung tissue 2- and 4-weeks post-Tam induction.

Article Snippet: To generate an I ER -SP-C I73T -Foxp3 DTR knock-in mouse line, we used B6.129(Cg)- Foxp3 tm3(Hbegf/GFP)Ayr /J purchased from The Jackson Laboratory (strain # 016958).

Techniques: Purification, Isolation, Quantitative RT-PCR, Expressing, Cell Stimulation, Staining, Enzyme-linked Immunosorbent Assay, Single Cell

Journal: Mucosal immunology

Article Title: Regulatory T cells protect against aberrant remodeling in a mouse model of pulmonary fibrosis

doi: 10.1016/j.mucimm.2025.12.004

Figure Lengend Snippet: Lung SP-C I73T T regs augment alveolar organoid growth supported by adventitial fibroblasts in vitro . (A) Schematic depicting tri-cellular organoid cultures generated using lung Tregs derived from SP-C I73T -Foxp3 eGFP mice 3–4 weeks after Tam induction, AT2 cells FACS sorted from SP-C CreER2 -Rosa26 Tdt mice, and adventitial fibroblasts isolated from Pdgrfa GFP mice; (B) Tricellular organoids were cultured for 2 weeks with activated CD3+ CD28+ Tregs replenished every week. Organoid cultures were imaged using EVOS FL auto and images were quantified using ImageJ an “analyze particle” macro with minimum area threshold set to1500 μm 2 . The experiment was independently repeated at least three times, each with three biological and three technical replicates. ****p < 0.00005 done by Ordinary one-way ANOVA; (C) Gradient plot from a previously published scRNA-seq dataset ( GSE234604 ) re-analyzed for expression of potential ligands made by immune cells as labeled (CD8+ T cell or Foxp3+ T regs) and cognate receptor expression on two fibroblast sub-populations (adventitial or alveolar) as labeled that are each increased in SP-C I73T mouse lungs at 2- and 4-week post Tam induction; (D) Bi-cellular organoids containing FACS purified TdTom+ AT2 and ScaI+ adventitial fibroblast were cultured with either 100 nM Areg, 200 nM Opn, or Areg+ Opn for 2 weeks. For comparison, tricellular organoids containing lung or spleen Tregs were prepared and co-cultured for 2 wks. (Left) Representative immunofluorescence staining of fixed wholemount organoid cultures co-stained for proSP-C and RAGE as labeled. Bars 50 μm; (Right) Live organoid cultures were images as in (B); (E) Representative immunofluorescence staining of fixed, frozen lung sections prepared form SP-C I73T -Foxp3 eGFP mice at 26 days post Tam induction stained for: (a) adventitial fibroblasts as CD39L1+ (white); (b) nuclei as DAPI+ (blue); (c) inherent Foxp3+ GFP+ fluorescence identifying Tregs (green); (d) composite overlay. Bars 100 μm. Insets 1 and 2 are magnified views of the boxed perivascular regions of lung with GFP+ Tregs and CD39L1+ adventitial fibroblasts.

Article Snippet: To generate an I ER -SP-C I73T -Foxp3 DTR knock-in mouse line, we used B6.129(Cg)- Foxp3 tm3(Hbegf/GFP)Ayr /J purchased from The Jackson Laboratory (strain # 016958).

Techniques: In Vitro, Generated, Derivative Assay, Isolation, Cell Culture, Expressing, Labeling, Purification, Comparison, Immunofluorescence, Staining, Fluorescence

Journal: eLife

Article Title: VPS9D1-AS1 overexpression amplifies intratumoral TGF-β signaling and promotes tumor cell escape from CD8 + T cell killing in colorectal cancer

doi: 10.7554/eLife.79811

Figure Lengend Snippet: ( A ) The Cancer Genome Atlas analysis confirmed that the highest VPS9D1-AS1 expression was predominantly in consensus molecular subtype 2 (CMS2)-type colorectal cancer patients. ( B ) CMS2 patients showed the lowest lymphocyte infiltration signal score. ( C ) Comparison of the percentages of CD4 + , CD8 + , and FOXP3 + T cells in cancer and cancer stromal tissues of OUTDO cohort. ( D ) Comparison of the number of CD4 + , CD8 + , and FOXP3 + T cells in cancer and normal tissues of the BJCYH cohort by immunohistochemistry assays. ( E ) Kaplan-Meier overall survival curves showed that CD4 + T and FOXP3 + T cells had no prognostic significance. ( F ) Proportions of CD4 + , CD8 + , and FOXP3 + T cell out of the total cells of Ca and STM tissues were compared according to the levels of VPS9D1-AS1 in BJCYH cohort. ( G ) Pearson correlation analyses investigated the relationships between TILs and TGF-β signaling in cancer stromal tissues of BJCYH cohort. p-Values were obtained by unpaired t nonparametric test ( A, B, C, D, F ), log-rank test ( E ), and Pearson correlation test ( G ). Data are shown as data points with mean ± SEM ( C, D, F ). *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: The primary antibodies against CD4 (Abcam, dilution 1:1000), CD8 (Santa Cruz, dilution 1:200), FOXP3 (CST, 1:800), and CK (Santa Cruz, dilution 1:200), respectively, stained for 1 hr at room temperature and then incubated with horseradish peroxidase-labeled mouse/rabbit secondary antibody for 10 min at room temperature.

Techniques: Expressing, Comparison, Immunohistochemistry

Journal: eLife

Article Title: VPS9D1-AS1 overexpression amplifies intratumoral TGF-β signaling and promotes tumor cell escape from CD8 + T cell killing in colorectal cancer

doi: 10.7554/eLife.79811

Figure Lengend Snippet: ( A ) Representative pictures of T cell infiltration (CD4, CD8, FOXP3) in colorectal cancer (CRC) tissues for VPS9D1-AS1 quantification. Tumor cells are marked by cytokeratin. ( B ) Representative pictures of CD8 + , CD4 + , FOXP3 + T cells, and TGFBR1 stained by immunohistochemistry (IHC) in the BJCYH cohort. ( C ) The overall survival curves depicting the percentage of surviving CRC patients stratified by the levels of CD8 + T cell infiltration in cancerous (Ca) tissues, cancer stroma (STM), and the Ca/STM ratio. ( D ) Ca/STM ratios of CD4 + , CD8 + , and FOXP3 + T cells were calculated to identify the difference between VPS9D1-AS1 negative and positive populations in the OUTDO cohort. ( E ) The numbers of CD4 + , CD8 + , and FOXP3 + T cells in cancer tissues of BJCYH cohort were compared between VPS9D1-AS1 negative and positive tissues. ( F ) Pearson correlation analyses investigated the relationships between T-infiltrating lymphocytes and TGF-β signaling in cancer tissues. Eight protein levels were investigated by multispectral fluorescence IHC assays in same samples, and fluorescence intensity of each protein level was transferred into quantitative data for Pearson correlation analyses. p-Values were obtained by log-rank test ( C ), unpaired t nonparametric test ( D, E ), and Pearson correlation test ( F ). Data are shown by mean ± SEM ( D, E ). * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: The primary antibodies against CD4 (Abcam, dilution 1:1000), CD8 (Santa Cruz, dilution 1:200), FOXP3 (CST, 1:800), and CK (Santa Cruz, dilution 1:200), respectively, stained for 1 hr at room temperature and then incubated with horseradish peroxidase-labeled mouse/rabbit secondary antibody for 10 min at room temperature.

Techniques: Staining, Immunohistochemistry, Fluorescence

Journal: eLife

Article Title: VPS9D1-AS1 overexpression amplifies intratumoral TGF-β signaling and promotes tumor cell escape from CD8 + T cell killing in colorectal cancer

doi: 10.7554/eLife.79811

Figure Lengend Snippet:

Article Snippet: The primary antibodies against CD4 (Abcam, dilution 1:1000), CD8 (Santa Cruz, dilution 1:200), FOXP3 (CST, 1:800), and CK (Santa Cruz, dilution 1:200), respectively, stained for 1 hr at room temperature and then incubated with horseradish peroxidase-labeled mouse/rabbit secondary antibody for 10 min at room temperature.

Techniques: Purification, Microarray, SYBR Green Assay, Reverse Transcription, Bicinchoninic Acid Protein Assay, Recombinant, Cell Isolation, Lysis, Northern Blot, Immunoprecipitation, Membrane, Chromatin Immunoprecipitation, Hybridization, Labeling

Journal: Cell Death & Disease

Article Title: TRIP13 promotes the expansion and immunosuppression of CD4 + Foxp3 + regulatory T cells by sustaining HAT1 stability

doi: 10.1038/s41419-025-08214-7

Figure Lengend Snippet: A Schematic diagram of experimental procedure. FCM analysis shows the proportion ( B , C , F , G ), number of colonic Tregs ( D and H ), and expression of Ki-67 ( E and I ) after LV5-NC or LV5- Trip13 treatment in WT and TNFR2 KO mice. The proportion ( J and K ) and number of colonic Tregs ( L ) after i.p. injection with Trip13 shRNA or LV5- Tnfr2 or control for 3 days were shown. The development of colitis ( M – O ), proportion ( P ), and number of colonic Tregs ( Q ) after colitis induction by TNBS in Foxp3 YFP-cre and Trip13 fl/fl Foxp3 YFP-cre mice were shown. Typical FCM plots display the proportion of gated cells. R , S The immunosuppression of Tregs in Trip13 fl/fl Foxp3 YFP-cre mice was determined. Representative FCM histogram ( R , the numbers indicate the proportion of replicating cells (%) and the division index), and the summary of the division index ( S ), were shown. Data are presented as means ± SEM from 3 mice per group and are representative of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. n.s. no significant differences.

Article Snippet: Foxp3 YFP-Cre (Cat. NO. C001467), CD4 Cre (Cat. NO. C001351), and CD45.1 mice were purchased from Cyagen Biosciences Inc. (Guangzhou, China).

Techniques: Expressing, Injection, shRNA, Control

Journal: Cell Death & Disease

Article Title: TRIP13 promotes the expansion and immunosuppression of CD4 + Foxp3 + regulatory T cells by sustaining HAT1 stability

doi: 10.1038/s41419-025-08214-7

Figure Lengend Snippet: Co-IP assays demonstrated the specific interaction between TRIP13 and HAT1 in MACS-sorted Treg cells ( A , B ) and HEK-293T cells transfected with Flag-TRIP13 and Myc-HAT1 plasmids ( C , D ). Proportion ( E and F ), number of colonic Tregs ( G ) and expression of Ki-67 ( H ) by colonic Tregs after i.p. injection with Trip13 shRNA or/and LV5- Hat1 (i.p.) for 3 days were analyzed by FCM. I , J MACS-sorted human Treg cells were used to transfect with the Trip13 shRNA or LV5- Hat1 in the presence of recombinant human TNF, and TNFR2 agonistic antibody for 7 days. Then the expression of Foxp3 was determined by WB ( I ), and the proliferation was measured by [ 3 H] TdR incorporation ( J ). Expression of CTLA-4 ( K , L ), and GITR ( M , N ) by Tregs after i.p. injection with LV5- Hat1 in control or TRIP13 cKO mice for 3 days were analyzed by FCM. “Control +” represents the group treated with vehicle control (e.g., LV5-NC). In contrast, “control -” indicates a baseline group without any viral transduction. Data (means ± SEM, A-H: n = 3 mice, I-J: n = 3 or 4 healthy donors) were representative of three separate experiments. Compared with the indicated group, *P < 0.05, ** P < 0.01, ***P < 0.001, n.s. no significant differences.

Article Snippet: Foxp3 YFP-Cre (Cat. NO. C001467), CD4 Cre (Cat. NO. C001351), and CD45.1 mice were purchased from Cyagen Biosciences Inc. (Guangzhou, China).

Techniques: Co-Immunoprecipitation Assay, Transfection, Expressing, Injection, shRNA, Recombinant, Control, Transduction

Journal: Cell Death & Disease

Article Title: TRIP13 promotes the expansion and immunosuppression of CD4 + Foxp3 + regulatory T cells by sustaining HAT1 stability

doi: 10.1038/s41419-025-08214-7

Figure Lengend Snippet: A Schematic diagram of experimental procedure. Naïve CD4 + T cells (Teffs, 4 × 10 5 cells/mouse) from Ly5.1 B6 mice (CD45.1 + ) alone or together with CD4 + Foxp3/YFP + cells (Tregs, CD45.2 + , 2 × 10 4 cells/mouse) were injected intraperitoneally into Rag1 −/− mice. The mice were treated with LV5- Trip13 , Hat1 shRNA, or vehicle control (i.p.) once a week, starting from 2nd day after cell injection, for 5 weeks. The mouse colon and spleen were harvested on week 8 after cell transfer. The proportion of Tregs (CD45.2 + ) in colonic CD4 + T cells before ( B , left panel) or 8 weeks after transfer ( B , middle and right panel), number of splenic Tregs ( C , D ), Foxp3 expression on colonic Tregs E were analyzed by FCM. F H&E staining of the colon, G Histological score, H Length of the colon, and I Body weight gain/loss (% of initial) were shown. Arrows indicated inflammatory cell infiltrates. Data (means ± SEM) shown in (n = 4 mice) were representative of three separate experiments. Compared with the indicated group, *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significant differences.

Article Snippet: Foxp3 YFP-Cre (Cat. NO. C001467), CD4 Cre (Cat. NO. C001351), and CD45.1 mice were purchased from Cyagen Biosciences Inc. (Guangzhou, China).

Techniques: Injection, shRNA, Control, Expressing, Staining

Journal: Cell Death & Disease

Article Title: TRIP13 promotes the expansion and immunosuppression of CD4 + Foxp3 + regulatory T cells by sustaining HAT1 stability

doi: 10.1038/s41419-025-08214-7

Figure Lengend Snippet: A Schematic diagram of experimental procedure. The proportion of Tregs (CD45.2 + ) in colonic CD4 + T cells before ( B , left panel) or 8 weeks after transfer ( B , middle and right panel), number of splenic Tregs ( C , D ), Foxp3 expression on colonic Tregs ( E ) were analyzed by FCM. F H&E staining of the colon, G Histological score, H Length of the colon, and I Body weight gain/loss (% of initial) were shown. Arrows indicated inflammatory cell infiltrates. Data (means ± SEM) shown in (n = 5 mice) were representative of three separate experiments. Compared with the indicated group, **P < 0.01, ***P < 0.001, n.s., no significant differences.

Article Snippet: Foxp3 YFP-Cre (Cat. NO. C001467), CD4 Cre (Cat. NO. C001351), and CD45.1 mice were purchased from Cyagen Biosciences Inc. (Guangzhou, China).

Techniques: Expressing, Staining